Therapeutic effects of different dosage regimens of Conbercept on clinical significant diabetic macular edema / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To observe the therapeutic effects of different dosage regimens of conbercept on clinical significant diabetic macular edema(CSME).<p>METHODS: Totally 65 patients with diabetes and CSME who were admitted to the Ophthalmology Department at Ganzhou People's Hospital between January 2019 and January 2020 were selected as the research subjects, and divided into observation group(<i>n</i>=33, conbercept 5+PRN regimen)and control group(<i>n</i>=32, conbercept 3+PRN regimen)using random number table method. Visual acuity test and optical coherence tomography(OCT)were carried out at 1, 2, 3, 6, 9, 12mo after treatment. Changes in the best corrected visual acuity(BCVA,LogMAR)and central macular thickness(CMT)were compared between the two groups. The mean injection times and complications in the two groups were recorded.<p>RESULTS: The BCVA was improved and CMT thinned in the two groups at 1, 2, 3, 6, 9, 12mo after treatment(<i>P</i><0.05). There was no statistically significant difference in BCVA and CMT between the two groups before treatment, and at 1, 2, 3, 6, 9, 12mo after treatment(<i>P</i>>0.05). The mean injection times in observation group was more than that in the control group \〖(5.81±0.54)times <i>vs </i>(4.19±0.41)times\〗(<i>P</i><0.05). In the early stage of postoperative follow-up, there were 30 times(23%)and 15 times(22%)of subconjunctival hemorrhage in observation group and control group, respectively(<i>P</i>>0.05). No other severe complications were observed in the two groups.<p>CONCLUSION:Both conbercept 3+PRN and conbercept 5+PRN are effective in the treatment of patients with diabetes and CSME. Both can significantly improve the patients' visual acuity and reduce CMT, with equivalent effect. The latter may increase the injection times but its adverse reactions are tolerable. In addition, it is safe and reliable.

Clinicopathological analysis of mucosa associated lymphoid tissue lymphoma secondary to Sjögren' s syndrome in salivary gland / 北京大学学报(医学版)

OBJECTIVE@#To analyze the clinicopathological characteristics of mucosa associated lymphoid tissue (MALT) lymphoma secondary to Sjögren' s syndrome (SS) (SS-MALT lymphoma) in salivary gland and to explore the value of the combined application of histopathological morphology, protein expression and molecular phenotype in pathological diagnosis and prognostic evaluation of SS-MALT lymphoma.@*METHODS@#Sixteen patients with SS-MALT lymphoma were collected from 260 patients who were diagnosed with SS in Peking University School and Hospital of Stomatology from January 1997 to December 2016. Twelve patients with non-MALT lymphoma secondary to SS (non-SS-MALT lymphoma) in salivary gland were selected as controls. The clinical data of the patients were retrospectively reviewed and analyzed. All the patients were followed up until December 20, 2019. Hematoxylin-eosin staining, immunohistochemistry, polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques were used to observe the histologic characteristics and to detect the manifestations of light chain restrictive expression, immunoglobulin (Ig) gene clonal rearrangement, chromosome translocation and gene abnormality, so as to evaluate their values in pathological diagnosis and prognostic evaluation.@*RESULTS@#The malignant transformation rate of SS to MALT lymphoma was about 6.15%, ranged from 3 to 240 months, during which 2 patients died due to high-level deterioration. Microscopically, the acini of the glandular tissue were atrophied and destroyed. The tumor cells dominated by central cell-like lymphocytes grew diffusely, destroying the epithelial islands. All SS-MALT lymphoma cases were positive in CD20 and Pax5. Half of them had the Ki-67 proliferation index of 10% or less, and half greater than 10%. 93.75% cases expressed AE1/AE3 protein, which showed the residual glandular epithelium. All the tumor cells were negative in CD3ε, and the plasma cells were detected by CD138 antigen. The light chain restrictive expression of κ and λ was 37.5% in SS-MALT lymphoma group. The positive detection rates of immunoglobulin heavy chain (IgH)-FR1, IgH-FR2, IgH-FR3, immunoglobulin kappa chain (IgK)-A, and IgK-B in SS-MALT lymphoma group were 33.3%, 53.3%, 33.3%, 20.0%, and 26.7%, respectively, and 93.3% when together used with IgH and IgK. The positive rates of the MALT1, IGH and BCL6 genes with dual color break-apart probes were 36.4%, 27.3% and 27.3%, and the detection rate of chromosome translocation and gene abnormality by applying the three probes was 72.7%.@*CONCLUSION@#There are no specific histological characteristics and protein phenotypes in the histologic diagnosis of SS-MALT lymphoma in salivary gland. The combined application of histopathological manifestations, immunohistochemistry, PCR and FISH techniques helps the accurate pathologic diagnosis of the disease. Although SS-MALT lymphoma is considered as an indolent lymphoma with a relatively favorable prognosis, the regular return visit and long-term follow-up should be conducted to detect the clues of recurrence and advanced deterioration.

Epigallocatechin-3-gallate Modulates MicroRNA Expression Profiles in Human Nasopharyngeal Carcinoma CNE2 Cells / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Epigallocatechin-3-gallate (EGCG) has exhibited antitumor properties in several types of cancers, including nasopharyngeal carcinoma (NPC), but the molecular mechanisms underlying this function remain incompletely understood. The aim of the present study was to characterize the global impact of EGCG on the expression of microRNAs (miRNAs) in NPC cells.</p><p><b>METHODS</b>Using microarray analysis, the alterations of miRNA expression profiles were investigated in EGCG-treated CNE2 cells. Furthermore, the target genes and signaling pathways regulated by EGCG-specific miRNAs were identified using target prediction program and gene ontology analysis.</p><p><b>RESULTS</b>A total of 14 miRNAs exhibited >2-fold expression changes in a dose-dependent manner after treatment with 20 μmol/L and 40 μmol/L EGCG. Totally 43, 49, and 52 target genes from these differentially expressed miRNAs were associated with the apoptosis, cell cycle regulation, and cell proliferation, respectively. A total of 66 signaling pathways, primarily involved in cancer development and lipid and glucose metabolism, were shown to be regulated by EGCG-specific miRNAs.</p><p><b>CONCLUSION</b>EGCG induces considerable alterations of miRNA expression profiles in CNE2 cells, which provides mechanistic insights into cellular responses and antitumor activity mediated by EGCG.</p>

The role of histone H3 phosphorylation at Ser10 in regulating proliferation and transformation of human nasopharyngeal carcinoma cells / 肿瘤

Objective: To explore the role of histone H3 phosphorylation at serine10 (Ser10) in regulating proliferation and transformation of human nasopharyngeal carcinoma cell line CNE1. Methods: The level of histone H3 phosphorylation at Ser10 was detected by Western blotting in the presence of epidermal growth factor (EGF). The wide-type histone H3 (H3WT) and mutant histone H3 (H3S10A) recombinant expression vectors were constructed, and then were transfected into CNE1 cells. The CNE1 cells stably expressing wide-type and mutant histone H3 were screened with blasticidin, and then the expression of histone H3 and EGF-induced histone H3 phosphorylation at Ser10 were detected in transfected cells by Western blotting. Cell counting kit-8 (CCK-8) assay and soft agar assay were employed to assess the effect of over-expression of wide-type and mutant histone H3 on proliferation and colony-formation of CNE1 cells. The expression levels of c-Jun, c-Fos and Bcl-2 were detected by Western blotting, and the activator protein-1 (AP-1) transcriptional activation were examined by dual-luciferase reporter gene assay. Results: EGF induced phosphorylation of histone H3 at Ser10 in CNE1 cells. The CNE1 cells stably overexpressing histone H3WT and mutant H3S10A were successfully established. The level of histone H3 phosphorylation at Ser10 induced by EGF was greatly decreased in mutant H3S10A CNE1 cells. As compared with the mock control tranfected with an empty vector, the abilities of proliferation and colony formation were obviously promoted in H3WT-overexpressing CNE1 cells stimulated by EGF, as well as the expression levels of c-Jun and c-Fos and transcriptional activation of AP-1 were significantly increased. However, no significant changes were observed in mutant H3S10A-overexpressing CNE1 cells. Conclusion: Histone H3, especially the Ser10 motif, might have an important role in regulating EGF-induced proliferation and transformation of CNE1 cells, which is relate with promoting c-Jun and c-Fos gene transcription and thereby enhancing AP-1 activity.

Effects of mitomycin C on membrane permeability and ultrastructure changes in human pterygium fibroblasts / 中华实验眼科杂志

Background Mitomycin C (MMC) has an inhibitory effect on the growth and proliferation of human pterygium fibroblasts,however,there is little literature about its influence on plasma membrane. Objective This study was to investigate the influence of MMC on the physical and chemical features and ultrastructures of plasma membrane. Methods Human pterygium tissues were obtained during the surgery.Human pterygium fibroblasts were primarily cultured and passaged using explant cultured method and identified by Vimentin staining.The third generation of cells were incubated to 96 well plate at a density of 5 × 103 cells/well,and 0,50,100,200,300 and 400 mg/L MMC was added in the culture well respectively to act for 12 hours.Cell viability was assayed using cell counting kit-8 ( CCK-8 ),and cellular apoptosis was detected using annexin V-FICT/PI.The changes of cell membrane structure were examined under an atomic force microscope.Malondialdehyde( MDA ) content in cell supernatant and level of lactate dehydrogenase ( LDH ) in extracellular fluid were detected to assess the lipid peroxidation level and permeability of cell membrane.Intracellular Ca2+ changes and membrane surface topography were assessed by Fluo-3/AM mark and flow cytometry( FCM ).This study was approved by Ethic Commission of Affiliated First Hospital of Jinan University.Informed consent was obtained from each patient. Results A lot of cells grew with the shape of spindle 1-2 weeks after culture.Positive response was seen in cultured cells for Vimentin.Growth and proliferation of the cells reduced 12 hours after action of MMC with the increase of MMC concentrations.The apoptosis rate of human pterygium fibroblasts was 4.2％,4.2％,5.4％,19.3％ and 25.8％ in 0,50,100,200 and 300 mg/L MMC groups respectively.Different degrees of abnormalities of cells membrane were found in various concentrations of MMC groups.The elevated contents of LDH and MDA in extracellular fluid were detected in various concentrations of MMC groups compared with the control group,and the LDH and MDA levels were gradually ascended as the increase of MMC concentrations,with a significant difference between any two groups(P＜0.05).The disturbance of intracellular Ca2+ homeostasis was also been seen after MMC acted. Conclusions MMC leads to the changes of physical and chemical features in human pterygium fibroblasts at a dose-dependent manner.Cell membrane may be the acting target of MMC.

Suppression of corneal neovascularization by culture supernatant of human amniotic epithelial cells in vitro / 中华实验眼科杂志

BackgroundCorneal neovascularization (CNV) is a common eye disease.The researches on the pathogenesis and treatment of CNV are focus in Ophthalmology.ObjectiveThis study was to investigate the effects of culture supernatant from human amniotic epithelial cells (AECs) on CNV in vitro and its mechanism.MethodsHuman AECs were obtained from a placenta and cultured in serum-free medium for 48 hours,and the supernatant was collected.The levels of interleukin-1 receptor antagonist (IL-1 Ra) and pigment epithelium-derived factor (PEDF) in the human AECs culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA).Rabbit corneal epithelial cells (CECs) were obtained and cultured in different concentrations of human AECs culture supernatant for 48 hours,serum-free medium was used as the control group.The expression of vascular endothelial growth factor (VEGF) mRNA and basic fibroblast growth factor (bFGF) mRNA in rabbit CECs were measured by quantitative real-time PCR (QRT-PCR).Human umbilical cord vein endothelial cells (UVECs) were cultured in the three mediums above,and the proliferation of human UVECs (absorbance value,A value) was tested by the cell counting kit 8 ( CCK8 ).Migration assay was performed by the wound healing method for the human UVECs.The membrane ultra-structure of human UVECs was examined under the atomic force microscope (AFM).ResultsCultured and passaged human AECs showed a positive response for keratin.The expression of VEGF mRNA (1.00±0.22) and bFGF mRNA (1.00±0.36) in rabbit CECs was suppressed by the human AECs culture supernatant,with a significant reduction in comparison with the serum-free DMEM group (2.98±0.46,2.55±0.48 )(P=0.001,0.002).The A value was significantly lowered in the human AECs culture group for 72 hours compared with the serum-free DMEM group ( 1.941 ± 0.036 versus 2.144 ± 0.059 ) ( P =0.000 ),and the bFGF-induced migration rate of human UVECs was strongly inhibited by the culture supernatant of human AECs in comparison with serum-free DMEM.The plasma membrane of human UVECs cultured with the human AECs culture supernatant was full of bumps,and decreased intercellular connection and cellular pseudopodia were found on the AFM image.The concentration of IL-1Ra was (153.56±0.36)ng/L and that of PEDF was (70.41 ±0.68 )μ,g/L in the human AECs culture supernatant.Nothing was deteched in serum-free DMEM group.Conclusions Human AECs culture supernatant suppressed the expression of VEGF and bFGF in CECs as well as the migration and proliferation of vascular endothelial cells.The effect may be associated with IL-1Ra and PEDF secreted by human AECs.These results suggest that human AECs may be a potential therapy for the inhibition of CNV.

Effects of antler powder on treatment of corticosteroid-induced avascular necrosis of the femoral head in rats / 中国组织工程研究

BACKGROUND: Long-term utilization of steroid hormone easily induces avascular necrosis of the femoral head (ANFH). Cornu Cervi Pantotrichum possesses bone growth factor and can promote wound healing and tissue repairing, which may has effect on treatment of ANFH. OBJECTIVE: To investigate therapeutic effect of antler powder on ANFH induced by corticosteroids in rats. METHODS: Totally 42 male wistar rats were randomly divided into the blank control, model and high-, moderate- and low-dose treatment groups. Exception those in the blank control group, all rats were intragluteally injected with dexamethasone at 30 mg/kg twice per week for 6 weeks to induce ANFH. Then the rats were treated with antler powder by oral gavage at 200, 400 and 800 mg/kg dosage, once per day for 60 days. Same volume of physiological saline was gavaged in the blank control and model groups. Serum lipid level and bone mineral density (BMD) of femur were measured, pathological change and expression of vascular endothelial growth factor (VEGF) were investigated. RESULTS AND CONCLUSION: Steroid hormone intervention resulted in obviously ANFH in rats. After treatment with antler powder, the degree of necrosis was significant reduced. Compared with the model group, BMD were increased, total cholesterol, triacylglycerol, and low density lipoprotein levels were decreased, and the VEGF expression increased in the treatment groups. The results suggest that antler powder has a positive curative effect on ANFH by promoting bone formation, fat metabolism and increasing VEGF expression.

Multiglycosides of Tripterygium wilfordii increase sperm apoptosis in male rats / 中华男科学杂志

<p><b>OBJECTIVE</b>To study the effect of multiglycosides of Tripterygium wilfordii (GTW) on sperm apoptosis in male rats and its possible mechanisms.</p><p><b>METHODS</b>Sixteen male SD rats were equally assigned to two groups to receive GTW and carboxymethylcellulose (CMC) intragastrically, both at 20 mg/(kg x d) for 6 weeks. Then the epididymal sperm was collected for the measurement of the apoptosis rate, sperm membrane lipid fluidity and the contents of NO, MDA and SOD by flow cytometry and spectrophotometric determination.</p><p><b>RESULTS</b>After 6 weeks of medication, the GTW group showed a significant increase in sperm apoptosis and contents of NO and MDA (P < 0.01) and a remarkable decrease in sperm membrane lipid fluidity (P < 0.05) and SOD content (P < 0.01) as compared with the CMC control group.</p><p><b>CONCLUSION</b>GTW can damage sperm membrane lipid peroxidation and sperm membrane structure, increase sperm apoptosis, and reduce sperm membrane lipid fluidity.</p>

In vitro study of the effects of copper ion on osteoclastic resorption in various dental mineralized tissues / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the effects of copper ion on osteoclastic resorption in various dental mineralized tissues.</p><p><b>METHODS</b>Osteoclasts were separated from long-limb bones of neonatal rabbits, and cultured with de-activated human tooth slices and glass slices. The cells in the experiment group were treated with (1 x 10(-14))-1 x (10(-4)) mol/L copper + 10% (volume fraction) fetal calf serum (FCS) + alpha-MEM, while the cells in control group cells were grown in 10% FCS + alpha-MEM. Osteoclasts on glass slices were stained by TRAP staining. The absorption pits on tooth slices were observed by inverted phase contrast microscope. The resorbing activity was evaluated with the concentration of calcium in the supernatant liquid of osteoclasts. The ratio between the concentration of calcium in the experiment group and control group was termed as the resorption index.</p><p><b>RESULTS</b>The isolated cells were multinuclear and TRAP positive in cytoplasma. Osteoclasts resorbed teeth slices first on the cementum and dentin. Compared with those on bone slices, the lacunae on the dental slices numbered less, with smaller volume and shallower in depth. Microscopy showed that the number and area of absorption pits formed on treated tissues were less than those on control tissues. The content of calcium in the supernatant liquid decreased in the concentrations of 1 x 10(-14) mol/L - 1 x 10(-4) mol/L copper, especially in the group of 1 x 10(-10) mol/L copper at 3rd day (P < 0.05) and 1 x 10(-4) mol/L, 1 x 10(-10) - 1 x 10(-12) mol/L copper at 7th day (P < 0.05). Their resorption index was lower than one.</p><p><b>CONCLUSIONS</b>Extracellular copper ion can inhibit osteoclastic resorption on dental slices.</p>

The genetic relation of four ethnic populations in China's Guizhou is revealed by sequence variations of mtDNA D-loop / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>Through maternal inheritance, to explore the genetic structures and relationships of Dong, Gelao, Tujia and Yi ethnic population in Guizhou of China.</p><p><b>METHODS</b>The mtDNA D-loop hypervariable segment I (HVS I ) in 108 samples of four ethnic populations were sequenced. Then, the nucleotide diversity was estimated and a phylogenetic tree was constructed by Neighbor-Joining method.</p><p><b>RESULTS</b>In the detected 497 bp fragments, 86 polymorphic sites were found, and 82 different haplotypes were identified. The phylogenetic tree of four ethnic populations showed: Yi, Tujia and Gelao clustered more closely than Dong did.</p><p><b>CONCLUSION</b>Yi and Tujia population are very closely related, the reason may be that they either originate from a common ancestry or frequently undergo the gene exchanges and admixtures. The genetic relationship between Tujia and Gelao population is nearer, perhaps because they have settled in the adjacent regions. Dong and Yi population show the farthest genetic relationship, this is probably due to their different historical origins and geographic segregation.</p>

Full face dermabrasion for acne scars / 中华整形外科杂志

<p><b>OBJECTIVE</b>Dermabrasion has been of great value in plastic surgery. Dermabrasion was developed for a specific indication; however, within a very short time, the concept of dermabrasion found wide applicability. This study was to investigate the application of dermabrasion in the treatment of acne scars.</p><p><b>METHODS</b>From Feb. 1996 to May 2004, a total of 110 patients with acne scar were treated with dermabrasion.</p><p><b>RESULTS</b>Postoperatively, the curative results were achieved in 45 cases; good results in 40 cases and effective results in 25 cases. The study revealed that the patients at 18-46 years of age have good results.</p><p><b>CONCLUSIONS</b>Dermabrasion is a good and safe technique to treat the scar of acne.</p>

Effect of genistein on the proliferation, differentiation and apoptosis of the osteoblasts / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of genistein on the proliferation, differentiation and apoptosis of the osteoblasts in vitro.</p><p><b>METHODS</b>The primary osteoblasts (OBs) were obtained from the rat calvaria and the cell line of osteosarcoma-UMR-106 served as control. The cells in the experiment group were grown in 10% (volume fraction) fetal calf serum (FCS) + alphaMEM + various concentrations of genistein. The control groups were grown in 10% FCS + alphaMEM. The growth of OBs was assessed by flow cytometry and MTT method. The differentiation of OBs was examined by the alkaline phosphatase (ALP) activity.</p><p><b>RESULTS</b>Flow cytometry analysis and MTT showed that genistein could prompt primary OB from stage G(0) (G(1)) to stage S, G(2) or M. By contrast, genistein had no effect on the cell cycle of UMR-106, but could induce its apoptosis. Additionally, the results of ALP activity showed that genistein stimulated the differentiation of primary OB.</p><p><b>CONCLUSIONS</b>Genistein can stimulate the proliferation and differentiation of the primary osteoblasts in some degree, and induce the apoptosis of osteosarcoma cells.</p>

Genistein inhibits the promotive effect of IL-1? on osteoclastic bone resorption / 北京大学学报(医学版)

Objective:To investigate the effect of genistein,a soybean-derived isoflavone,on thestimulating effect on bone resorption of IL-1?.Methods:The osteoclasts(OCs)were isolated with themethods of Yu Shifeng.The rat calvaria were cultured as an organ.The cells in the experiment weregrown in four respectively:Control(without genistein or IL-1?),10~(-6) mol/L genistein,10 ?g/L IL-1?and 10~(-6) mol/L genistein+10 ?g/L IL-1?.The area of bone resorption,the concentration of Ca~(2+) in thesupernatant liquid of OCs cultures and mice calvaria were tested.The contents of acid phosphatase(ACP)were also examined by biochemistry method.The index of bone resorption was counted as the ra-tio of the experiment average and control ones,which indicated the increase in bone resorption when itwas above 1.0.Results:The area of bone resorption of 10~(-6) mol/L genistein+10 ?g/L IL-1? increasedcompared with that of 10~(-6) mol/L genistein,while the concentration of Ca~(2+) in the supernatant liquid ofOCs cultures decreased significantly.The index of bone resorption of 10~(-6) mol/L genistein+10 ?g/L IL-1? lied between 10~(-6) mol/L genistein and 10 ?g/L IL-1?.In the organ culture,there was no differencein the content of ACP among all the groups,The index of bone resorption of 10~(-6) mol/L genistein+10?g/L IL-1? was below that of 10?g/L IL-1?,but both were above 1.0.The index of bone resorptionwas below 1.0 in the group of 10~(-6) mol/L genistein.Conclusion:Genistein can suppress obviously thebone resorption simulated by single IL-1?.

The investigation of tracheas transplantation by wrapping in a muscle flap / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the possibility of tracheas transplantation by wrapping it in a muscle flap.</p><p><b>METHODS</b>With a dog model, a number of tracheas were separately wrapped in the unilateral sternocephalic muscle flap and the bilateral sternohyoid-sternothyroid muscle flap, and placed in the original site. The tracheas autografting was used as a control. The viability was evaluated by the examination of fiberoptic bronchoscopy, histopathology and microangiography, the measurement of tracheal mucosal blood flow and the calculation of survival rate and percentage of patency.</p><p><b>RESULTS</b>The submucosal blood flow of the transplanted tracheas was detected in the unilateral sternocephalic muscle flap group and the bilateral sternohyoid-sternothyroid muscle flap group 1 week after the surgery and gradually reached the level close to the normal in 4 weeks, while the vascular ingrowth was also shown from the wrapped muscle flap into the transplanted tracheas by using a microangiography technique. The histopathological examination demonstrated that the structure of the transplanted tracheas was quite same as the original one and its inner surface was also covered with pseudostratified columnar ciliary epithelia. However, in the control group, the mucous membranes turned black one week after the transplantation and all dogs died from the graft necrosis.</p><p><b>CONCLUSION</b>The tracheas wrapped in a muscular flap could survive well for a long time.</p>

The beneficial effect of genistein on mandible bone metabolism in ovariectomized rats / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To detect the effect of genistein on mandible metabolism in ovariectomized rats.</p><p><b>METHODS</b>Forty 12 week-old female SD rats were randomly assigned to four groups: (1) sham operated; (2) ovariectomized; (3) ovariectomized and treated with estradiol; (4) ovariectomized and received genistein, 45 mg/kg body weight per day. After 12 weeks, bone mineral density (BMD), serum level of alkaline phosphatase (ALP), acid phosphatase (ACP), osteocalcin, IL-1beta, TNF-alpha, IL-6 and calcitonin (CT) were evaluated. In addition, the serum estradiol and the weight of uteri were also examined to indicate the side effect of genistein to the uteri.</p><p><b>RESULTS</b>Ovariectomized animals had a significant decrease in BMD, and increased serum level of ALP, ACP, IL-1beta and osteocalcin compared with sham rats. After treated with genistein, BMD and the serum level of ALP, ACP, osteocalcin increased significantly, while the serum level of IL-1beta and TNF-alpha decreased. Especially, the increase of ALP and osteocalcin was higher than that of estradiol-treated animal. Additionally, the uterus weight index and the serum estradiol in genistein-treated rats were lower significantly than those of estradiol-treated rats.</p><p><b>CONCLUSIONS</b>Genistein can improve the mandible bone metabolism as well as its effect on femur through the promotion of bone formation and the prevention of bone resorption with slight side effect. Genistein provides an additional viable way to therapy for osteoporosis in the jaw bones.</p>